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This project work stemmed from the need to assess the marketing structure, conduct and performance of the fruit in selected markets of Lagos state by using structured questionnaire which was administered to apple fruit marketers in the study area. Isoelectric Focusing IEF Ionic buffers called ampholytes and an applied electric field are used to generate a pH gradient within a polyacrylamide matrix. Some of the major challenges that medicine and related health sciences face are also outlined very briefly at the end of Chapter Carboxyl groups, amino groups, and phosphate esters, whose second dissociation falls within the physiologic range, are present in proteins and nucleic acids, most coenzymes, and most intermediary metabolites. The net charge on an amino acid�the algebraic sum of all the positively and negatively charged groups present�depends upon the pKa values of its functional groups and on the pH of the surrounding medium.